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貨號(hào) | 品牌 | CAS | 規(guī)格 | 銷售價(jià)(RMB) | 折扣價(jià)(RMB) | 備注 | 數(shù)量 |
ABT2200-200μL | Abbkine | 無(wú) | 200μL | 1698 | 1698 | 無(wú) | |
ABT2200-50μL | Abbkine | 無(wú) | 50μL | 578 | 578 | 無(wú) | |
ABT2200-200μL×5 | Abbkine | 無(wú) | 200 μL×5 | 5816 | 5816 | 無(wú) |
產(chǎn)品英文名稱 | Anti-TAP Tag Monoclonal Antibody (4H2) |
免疫原 | 重組蛋白 |
宿主 | 小鼠 |
應(yīng)用 | WB |
實(shí)驗(yàn)建議 | 最佳的工作稀釋比例需要客戶摸索優(yōu)化。建議的起始嘗試的稀釋比為:WB:1:5000-1:10000。 |
克隆性 | 單克隆 |
純化工藝 | 通過(guò)使用特異性免疫原的親和層析,從小鼠腹水中純化抗體。 |
產(chǎn)品形式 | 液體溶液 |
儲(chǔ)存緩沖液 | PBS緩沖液(pH 7.4),含有0.5%BSA(穩(wěn)定劑),0.02%疊氮化鈉(防腐劑)和50%甘油。 |
保存建議 | 自發(fā)貨之日起,-20°C可穩(wěn)定保存1年。為最大限度的避免損失,請(qǐng)?jiān)诖蜷_(kāi)管蓋之前融化抗體并離心。我們建議使用前分裝以避免反復(fù)凍融。 |
運(yùn)輸條件 | 藍(lán)冰運(yùn)輸 |
警告 | 本文列出的產(chǎn)品僅供研究使用,不適用于人類或臨床診斷。我們產(chǎn)品所推薦應(yīng)用,不是建議使用我們的產(chǎn)品去違反任何專利或許可證。對(duì)于使用本產(chǎn)品可能發(fā)生的專利侵權(quán)或其他違規(guī)行為,我們不承擔(dān)任何責(zé)任。 |
背景 | The TAP (Tandem Affinity Purification) method is an affinity purification method for the isolation of TAP-tagged proteins along with associated proteins. The TAP tag historically consists of a calmodulin binding peptide (CPB, a tobacco etch virus (TEV) protease cleavage site, and Protein A. However, additional tag combinations have been used with the TAP method including the combination of FLAG tags and HA tags. The TAP method permits the identification of proteins interacting with a particular target protein without any prior knowledge about the function, activity, or composition of the interacting proteins. The TAP tag has been especially useful and deployed with Yeast Tap-tagged ORF clones. These clones contain genomic fusions of the TAP construct and are extremely useful for determining natural protein interactions and expression level variations based on physiological changes. |
別名 | TAP tag; Tandem Affinity Purification; Tandem Affinity Purification Tag |
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